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Archival RNA from diagnostic submissions obtained during investigations of HeV in horses in Australia in 2011 was tested and results then correlated with the original diagnostic results obtained from qPCR assays (Table 3).

This study could be performed retrospectively, since sequence submission dates are easily obtained from public databases.

Sequence information from PRRSV ORF5 and related source-herd demographic information were obtained from diagnostic submissions to the Animal Health Laboratory, University of Guelph.

The B. trehalosi isolates were field strains obtained from diagnostic submissions and the M. haemolytica isolate was a proprietary leukotoxin positive isolate.

Furthermore, retrospective data on sample submission and circulating FMDV serotypes were obtained from publically available annual reports of WRLFMD, Pirbright [ 26] and from GenBank®, National Centre for Biotechnology Information (NCBI) [ 27].

During a 60-month period (January 2006 to December 2010), the day and hour of submission of all consecutive BALF samples obtained from patients suspected of VAP were recorded.

Additional clinical information was obtained from the original authors' submission, the soft file in entry GSE4573, and from supplementary data in the published paper.

Informed consent was obtained from each subject by submission of a questionnaire sheet.

NCI-60 panel genomic DNA were obtained from the National Cancer Institute (after submission of a project involving a formal request) and genomic DNA from 34 CRC cell lines from Sir Walter Bodmer's laboratory [18].

Complete data files can be obtained from Gene Expression Omnibus under the submission ID GSE55118.

Three of the authors are employees of Shire Pharmaceuticals, and approval for publication was obtained from Shire Pharmaceuticals before submission of this manuscript.