FMY and ODA designed CP primers, ARA, NB, EJC, KRD, JH, HH, NH, DT, DMT, CT, and WZ performed PCR amplification, purification of amplicons and their submission for sequencing, alignment of sequences and design of GSPs; ODA, DCD, and CCC sequenced PCR amplicons.
The current effort on the adoption of a data-sharing standard, such as the Minimum Information about a high-throughput SeQuencing Experiment (MINSEQE), aims to provide guidelines on submission of sequence data to the public repository [ 28].
Submission of sequence data to VIROME is initiated through the web-application interface.
Automated gene-calling and functional annotation (EC number, COG, Pfam, TIGRfam, KEGG, Metacyc) was carried out through submission of sequence assembly to Joint Genome Institute's Integrated Microbial Genomes Expert Review (IMG/ER) [ 34].
Sections are organized to address the various phases of publication (e.g., About Your Manuscript, Manuscript Preparation, Manuscript Submission, Publication Sequence).
Determination of the presence of a signal secretion peptide or transmembrane helices was accomplished by the submission of sequence peptides to the SignalP server [ 32] or TMHMM server [ 33], respectively.
In addition, because molecular epidemiologic studies and phylogenetic analysis of DENV strains will provide more informed insight into origins and distribution of DENV across Africa, submission of sequence data for as many samples as possible should also be encouraged.
It is a well written report, however, I have the following observations on the current submission: The sequence of the work up is not clear: was the MRI done first; or were urinary catecholamines and metanephrines, aldosterone-renin ratio and serum cortisol determined first?
However, submission of sequences to upcoming versions of mirBase require adherence to very strict criteria imposed upon high-throughput sequencing data.
Amplified cDNA products were gel-purified prior to submission for sequencing.
List of specimens and HmtDB identifiers, obtained after submission of sequences to the HmtDB, are reported in Supplementary Table 2 SampleData.
