In this experiment, the photosynthetic ETR was determined in the leaf discs exposed to air, but the respiratory rate was determined in the leaf discs submerged in solution.
Initial experiments are also described whereby the local conductivity sensor is submerged in solutions of various concentrations of different salts commonly found in mammalian cells.
Microfabricated Ag/AgCl electrodes also suffer from potential instability and reduced lifetime when submerged in solutions less than 3 M chloride.
Afterwards, eye cups were transferred to PBS to remove formaldehyde and submerged sequentially in solutions of 10%, 20% and 30% sucrose for at least 1 h each.
To establish the force versus pCa relationship, fibers were submerged in a solution containing no added calcium (pCa 8.5), followed by sequential exposure to 13 different calcium solutions, namely pCa 6.0, 5.80, 5.75, 5.70, 5.65, 5.60, 5.55, 5.50, 5.40, 5.30, 5.20, 5.20 and 5.0.
To assay catalase activity, gel strips were washed three times in distilled water for 15 min, submerged in a solution of 3 mM H2O2 for 25 min, rinsed briefly with distilled water, and developed in a solution of 1% w/v K3Fe CN 6 and 1% w/v FeCl3 for 10 15 min until clear bands appeared [35], [36].
The isolated tissues were submerged in RNAlater solution overnight at 4 °C and then, stored at –80 °C.
Place the gel in 0.125M HCl so that the gel is completely covered (submerged) in the solution.
Then a thin layer of aluminum oxide is deposited on top and the plastic film is submerged in a solution containing an insulating polymer.
Afterwards, the gold plates were submerged in thiol solution of interest at room temperature to form a stable coating on the surface with a similar thickness.
Subsequently, the sample is submerged in a solution of Cu+ cations in methanol (4 mL, [Cu+] = 75 mg/mL) for 5 min at room temperature to trigger the CdSe→Cu2Se CE transformation.
