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Average responses from the six subjects were computed, using circular statistics for the phase data [20] and the Rayleigh test for significance with p<0.01.
Analyses of data from individual subjects were computed using the general linear model with local autocorrelation correction (25).
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The socioeconomic status of the families of the subjects was computed using the method recommended by Oyedeji in his study of the socioeconomic and cultural background of children hospitalized in Ilesa, Nigeria.
The questionnaire included a validated semi-quantitative food frequency section listing 165 food items commonly consumed in the study population, from which average daily intake of calories and roughly 100 nutrients and non-nutritive ingredients per subject were computed using the Singapore Food Composition Table [14] which we developed in conjunction with the cohort study.
Following PCA, factor scores for each subject were computed using SAS Enterprise Guide 4.3, as previously reported (Huffman et al., 2009).
GC content was computed using CODONW [ 26].
Between- and within- (between-days) subject variance components were computed using a one-way random effect model, and intra-class correlation coefficients (ICC) were calculated from the variance components as standardized measures of reliability.
After global normalization of data from separate sessions, single-subject statistical contrasts were computed using a parametric general linear model (Buchel et al., 1998; Friston et al., 1998).
The thalamo-striatal, striato-pallidal and pallido-thalamic tracts in each subject and each hemisphere were computed using the same criteria applied for the reconstruction of cortico-striatal and thalamo-striatal connections.
As a consequence, scaled scores for seven subjects younger than the age of 16 were computed using normative data for subjects aged 16 years.
For example in scheme (b), P Q j = 1 | L i) and P L i) were computed using the subjects recruited online (n = 100) and used to classify subjects recruited at the clinic (n = 62).
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