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This resulted in an artificial cohort consisting of 25,640 subjects ("HNSCC artificial cohort"), an artificial cohort consisting of 17,310 subjects ("TBI artificial cohort") and an artificial cohort consisting of 19,086 subjects ("CHIP artificial cohort").
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Cells were serum starved as described above, re-entered into the cell cycle by the addition of medium containing 10% FBS, and cultured for 14 h before being subjected to ChIP analyses (ChIP assay kit; Upstate Biotechnology).
Half of the culture was utilized for microarray expression profiling in which the transcriptome of the transcription factor overexpression strain was compared to an empty vector control, while the other half was subjected to ChIP-chip analysis.
"TaleSpin" (1990) Reallllly feelin' this song, and while we're on the subject, remember "Chip n' Dale Rescue Rangers"?
Forty-eight hours after transfection, cross-linked chromatin was prepared and subjected to ChIP assay.
Following formaldehyde cross-linking, chromatin was isolated and subjected to ChIP and amplification of captured DNA sequences.
Among all the up-regulated genes subjected to ChIP analysis, gadd45b showed increased RNAPII occupancy at the 3' end in the fogsk8 mutant embryos.
The recovered complexes after the secondary immunoprecipitation were eluted with the elution buffer and then subjected to ChIP assay, as described.
To investigate the effect of LMO3 on binding of HEN2 and HES1 to putative HES1-binding sites and E-box, Neuro2a cells were transfected with Myc-HES1 or FLAG-HEN2 together with or without HA-LMO3 expression plasmid and subjected to ChIP assay.
HeLa and SiHa cells were subjected to ChIP with the EZ-ChIP Assay kit (Millipore).
The cells were subjected to ChIP assay by using ChIP Kit (Abcam, Inc., Cambridge, MA).
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CEO of Professional Science Editing for Scientists @ prosciediting.com