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Gray box indicates subjective light period and black box indicates dark period.
White box indicates light period, black box indicates dark period, and gray box indicates subjective light period.
On day 6 of the culture, cells were either kept in a 12 hour LD cycle or transferred to DD. Media was collected every four hours for a 24-hour period; sampling began 4 hours after lights on for cells in LD, or 4 hours after the beginning of the subjective light period for cells in DD.
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The experiment was started 2 h before (subjective) dusk and leaves were sampled at time 0 (ZT14) and after 2 h (to coincide with (subjective) light-dark transitions) and then every 4 h until 58 h (ZT72) (Figure 1).
The phase delay and advance of the temperature rhythms induced by single light pulses 4 or 10 hours after subjective lights off were similar in offspring of both control and CPS exposed mothers (Figure S1).
We analyzed the cycling of the clock protein PDP1ε in the major classes of clock neurons by IF. Figure 4 shows the staining index (SI) for PDP1 ε at CT0, CT6, CT12, and CT18 (circadian time, CT0 = subjective lights ON, CT12 = subjective lights OFF) during day 2 and 5 in DD.
In his own not easily understandable words, possibly clearer in the original German: Objective is the fractured landscape, subjective the light in which — alone — it glows into life.
During the subjective day, light has little or no effect on the resetting of the clock.
Pupil exam was normal with a 10% subjective reduction in light brightness OS.
During the subjective day, when light is ineffective in resetting overt rhythms, a very small fraction of the transcriptome changed in response to light.
But that impressionistic treatment – aimed at catching the subjective experience of light, fleeting fragments of perception – isn't at the heart of Knight's achievement.
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