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The model was subjected to further refinement using the md_refine.mcr macro of YASARA and to 1 ns constant temperature (300 K) and pressure (1 bar) molecular dynamics (MD) simulations using the AMBER03 force field.
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Particles in each class were sorted into two independent stacks and subjected to further projection-matching refinement.
A few top fits between the protein and the peptide conformations are selected as the predictions that may be subject to further refinement.
[Note: this provision remains subject to further refinement and discussion] For any public employee plan that is "at-risk" as defined by federal laws governing private pension plans, the government employer will be required to prospectively modify its employee pension benefits in such a way that will lift the plan out of "at-risk" status within XX years.
This interpretation may be subject to further refinement given that certain cell types in which fusion is compromised as a result of mitofusion 2 (mfn2) deletion show less impressive changes in mitochondrial ultrastructure than those described here and are actually somewhat larger than their mfn2+/+ counterparts.
The final snapshot at the end of the simulation was selected from the 2 ns and subjected to further energy minimization for fine refinement.
A total of 1000 structures were calculated, and the 200 lowest-energy structures were subjected to water refinement and further analysis by PROCHECK_NMR; 80.5% of the V domain residues were in the most favorable regions of the Ramachandran plot, 18.2% in additional allowed regions, and 1.3% in generously allowed regions.
All authors contributed to further refinement of the first draft and the revised manuscript.
Nevertheless, we recognize that implementation of these workflows will lead to further refinement and expansion.
If they fail, they are subjected to feature refinement.
Two of them were combined (13,219 in total) and subjected to structural refinement by RELION.
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CEO of Professional Science Editing for Scientists @ prosciediting.com