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Finally, the value of the constant parameter (const) was subjected to estimation.
The homogenate was subjected to estimation of malondialdehyde (MDA) [ 17], reduced glutathione (GSH) [ 18], and superoxide dismutase (SOD) levels [ 19].
The calculation time within individual estimation was principally dependent on the number of experimental data points and on the number of parameters subjected to estimation, and it varied between a few and over a dozen minutes.
Alignments in blocks with fixed sizes (50 bp to 1 kb) and fixed intervals (500 bp to 5 kb) were extracted and subjected to estimation of τ and θ using Rannala's MCMC framework (Rannala and Yang 2003), assuming the uniform model [model (1) described earlier].
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Esophageal tissues were further subjected to estimations of TBARS, GSH, catalase, and SOD.
If the model is deemed inadequate, a new tentative model is identified, which is also subjected to parameter estimation and model verification.
Among 272 accountable MSL spacecraft surface samples subjected to the estimation of spore burden using NSA, 22 samples (~8% of the 272 MSL surface samples) showed the presence of at least one spore.
Each micro-clade was subjected to tMRCA estimation using BEAST.
The saliva and sera from the blood samples were subjected to glucose estimation.
Later, stomachs were subjected to the estimation of catalase, superoxide dismutase, malondialdehyde, mucin, total protein, and nonprotein sulphhydryl contents following standard procedures.
The samples were withdrawn at regular intervals, centrifuged at 10,000 rpm for 10 min and the supernatant was subjected to sugar estimation.
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