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To determine if TBX3 could promote differentiation in RD cells, cells overexpressing TBX3 were subjected to differentiation conditions.
RD cells were grown to confluence, transiently transfected with shEZH2, and subjected to differentiation conditions for 2 days prior to immunofluorescence with MyHC antibodies.
Clones were subjected to differentiation analysis detailed above.
The other chip was subjected to differentiation medium (specified below) on day 2, whereby medium was changed every third day until day 9 when the chip was fixed and subjected to staining.
Cells were allowed to expand for up to two weeks and subjected to differentiation into contracting myotubes for 5 days in the presence of DMEM supplemented with 0.5% horse serum, Myogenic cultures containing mononuclear cells and/or myotubes were subsequently Xgal stained and photographed.
To confirm our hypothesis, RH30 cells were subjected to differentiation.
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Ikkα-deficient keratinocytes do not express terminal differentiation markers and continue to proliferate even when subjected to differentiation-inducing stimuli.
hMSC were transfected with a library of miRNA inhibitors and subjected to osteogenic differentiation by incubation in differentiation media.
The greater collagen synthesis and mineralised matrix per field exhibited by BM-MSCs compared to AD-MSCs subjected to osteogenic differentiation indicate not only improved osteogenic differentiation in the former but also greater capacity for extracellular matrix synthesis under the same culture conditions.
Furthermore, increased osterix expression by MSCs subjected to osteogenic differentiation is considered to be an indicator of differentiation in canines [ 12].
C2C12 cells were subjected to the differentiation and myotube formation by culturing in the differentiation medium composed of DMEM (Gibco BRL, Grand Island, NY, USA) and 10% HS (Gibco BRL, Grand Island, NY, USA), according to ATCC recommendations.
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