Thresholded images were subjected to image calculation procedures designed to highlight image elements (pixels) containing (green) fluorescence associated with the tracer protein; all other sources of fluorescence were visualised in the final images only if no green fluorescence was detectable in that pixel.
Means from two independent experiments were subjected to SEM calculation, student's t test using PAST (http://folk.uio.no/ohammer/past/).uio.no/ohammer/past/
The SSEA-1 positive cell numbers were subjected to the calculation of "cell population doubling time" with the equation Yend = Ystart×2 (t/T).
Genes with highly divergent sequences were not subjected to Ka/Ks calculation as a full-length sequence alignment with their respective Muguga counterpart was not obtained.
To estimate the evolutionary dates of the segmental duplication events, Ks was used as the proxy for time and the conserved protein-coding genes flanking the PLD gene pairs were thus subjected to Ks calculation (Table 3).
The conformers with Boltzmann-population of over 1% were subjected to ECD calculations.
The structures of mutant enzymes, at equilibrium, were subjected to docking calculations with selected PAHs.
For demonstration purposes, the chemical structures for a subset of the Sutherland datasets [36] were subjected to descriptor calculations for selected CDK descriptors and are available in QSAR-ML and archived Bioclipse projects at the QSAR-ML website [27].
To incorporate statistical fluctuations, a total of 30 configurations were generated through classical molecular dynamics simulation with water molecules and were subjected to FMO4 calculations at the MP2 level.
Each of the three ligands was immersed in an explicit water solvent, geometry-optimized by classical MM and QM/MM methods, and subjected to FMO calculations with 2-, 3-, and 4-body corrections under several fragmentation options.
Papers that did not provided the total number of observations (n) were subjected to mathematical calculations.
