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The sample #3 with off-target mutagenesis, together with randomly selected sample #2 and #18 were subjected for analysis.
An aliquot of the DNA was subjected for analysis using the Bioanalyzer (Agilent technologies) and was confirmed for no degradation of the DNA.
All the samples were then vortexed for 1 min and centrifuged at 3600 g for 10 min and further subjected for analysis.
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The samples were then filtered and subjected for AAS analysis.
Both input and immunoprecipitated proteins were subjected for immunoblotting analysis.
All the variables were measured thrice and their mean was subjected for statistical analysis.
Data from these subjects were subjected for further analysis.
The remaining clusters (CRF02_AG cluster 1 and 3 5) consisted of too few sequences to be subjected for demographic analysis.
A total of 94 individuals including 51 males were subjected for the analysis (Table 1).
The Rif1 homologues were subjected for phylogenetic analysis using ClustalX neighbour joining algorithm [ 40].
Thereafter mice were killed and pancreata were subjected for histological analysis.
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