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One μl of each sample was subjected (flow rate 3 μl/min) onto reverse-phase in-chip column (40 nl capacity) for 10 minutes under isocratic buffer (5% acetonitrile in 0.1% TFA).
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Then the cells were subjected to flow cytometric analysis with a flow cytometer (model BD LSR II, BD Bioscience, San Jose, CA).
Cells were subjected to flow cytometry on a FACSCaliber Flow Cytometer (Becton Dickinson) to determine percent of apoptotic cells.
Cells were then subjected to flow cytometry on a Cytomics FC500 flow cytometer (Beckman Coulter).
Cell suspensions were subjected to flow cytometry analyses as previously described using a LSRII flow cytometer [ 14].
SiRNA-transfected L929 cells were subjected to flow cytometry analysis 72 h post transfection.
Single cell suspensions prepared spleen and blood were subjected to flow cytometry analysis as described previously51.
Cells on Day 0 and 11 were subjected to flow cytometry analysis.
These samples were subjected to flow number testing.
After 14 days, secondary tumors were removed, weighed, and subjected to flow cytometry to identify GFP+ endothelial cells.
Suspensions of flocculated aggregates subjected to flow present two distinct typical fragmentation mechanisms: cleavage and erosion.
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CEO of Professional Science Editing for Scientists @ prosciediting.com