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In particular the scale authors suggest that the MSIS-29 should be subjected to validation with newer psychometric methods such as Rasch analysis [ 18, 19, 21].
The database was doubly entered by scientific initiation students, within the Statistical Package for the Social Sciences (SPSS) for Windows version 16.0 (Chicago, IL, USA) and was subjected to validation with the help of statistical package Epidata.
This framework presents validation as a five-phase process that starts with the often overlooked phase 'Is the simulation subject to validation?'.
All the laboratory tests were subject to validation using National External Quality Assurance Schemes.
Subsequently, 15 genes were subjected to validation by qRT-PCR.
These genes were subjected to validation by real-time PCR.
Fourteen randomly chosen SNPs, which induce amino acid changes in the CDS region, were subjected to validation using PCR and Sanger sequencing with larger numbers of SL and BL chickens.
When visible bands were detected, the cDNA samples were further subjected to validation PCR (validation set in Supplementary Table 3).
The development of several measures to elicit patient report of hospital stay (PJHQ & ERS) [ 11, 12] and patient rated clinical change (PGCI & GROC) [ 13, 37] have also been subject to content validation with patients to understand the meanings they ascribe to the questions posed.
Given the inherent difficulty in controlling for off-target effects in any knockdown experiment performed using a single siRNA, the results presented here should be subjected to independent validation with use of a second siRNA.
Peptide-spectrum matches (PSMs) were subjected to statistical validation with the PeptideProphet algorithm (TransProteomic Pipeline - TPP v4.3) using the accurate mass model option and the semi-supervised approach [ 21].
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