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(Figure 3D) when hESCs were subjected to this protocol.
While these changes were similar to those we have previously reported from ES cells subjected to this protocol [5], the expression of Six3, another EFTF, was somewhat different in the iPSCs than what we have observed in the ES cells.
However, many have found it difficult to reproduce this protocol, and researchers who have succeeded in making mice drink the antibiotics ad libitum have experienced increased baseline morbidity and mortality among some strains and genotypes of mice subjected to this protocol (W. Garrett, personal communication).
Three complete sets of these haustoria (of all four species) were successively subjected to this protocol.
As controls for secondary antibody background and autofluorescence, slides were subjected to this protocol omitting the primary antibody.
A total of 601 patients were subjected to this protocol, which was amended after 502 patients to allow for a higher insulin dose.
As of generation F12, a subsample of three males and three females from each Line C family were subjected to this protocol, providing a continuous control baseline moving forward.
Our results demonstrate that turbot embryos can be subject to this cryoprotectant protocol without deleterious effect on the hatching rate.
Furthermore, we demonstrated that mice subjected to this depletion protocol acquire a germ free-like phenotype.
In order to fully characterize the differentiated GBM cells subjected to this dedifferentiation protocol, we first checked by RT-qPCR the expression of several stem and differentiation markers in NS-derived cells and their differentiated counterparts.
But the bystanders subject to the protocols of both groups were innocent and now are dead.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com