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The membrane was then subject to staining with anti-Kv1.3 antibodies to locate the protein band.
Note: the surface is still subject to staining and damage at this point, and care should be taken to thoroughly wet and neutralize the entire area to avoid boot prints, splashed residue marks and other man-made blemishes.
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Cells were treated with 800 μmol/L H2O2 for the indicated time, and then the TIF was isolated and subjected to staining with anti-PCNA antibody.
Hearts embedded in paraffin were cut into 5-µm slice, five to six sections from apex to base were subjected to staining.
The other chip was subjected to differentiation medium (specified below) on day 2, whereby medium was changed every third day until day 9 when the chip was fixed and subjected to staining.
Biopsy specimens were embedded in paraffin and subjected to staining with hematoxylin and eosin.
Cells were then counted and subjected to staining for cell sorting.
Precipitated proteins were subjected to SDS-PAGE and the gel was subjected to staining with colloidal blue.
Briefly, tissue sections were thawed on pre-coated sylane slides and subjected to staining and immunostaining protocols.
To confirm that an equal amount of proteins were applied to each lane, the blots were subjected to staining for β-actin (Oncogene Science) as a control.
(A and B ) Coronal sections of adult Sema5A lacZ/lacZ mice were subjected to staining for Plxna2 mRNA (purple) or nuclear β-galactosidase (blue) by X-gal.
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CEO of Professional Science Editing for Scientists @ prosciediting.com