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Only the compounds that selectively protect TrkB expressing cells but not parental cells without TrkB from apoptosis were subjected to next round functional analysis.
Three small libraries were constructed and subjected to next generation sequencing.
Genomic DNA was isolated from each sample and subjected to next generation sequencing.
Resulted top scoring molecules (10%) were subjected to next round of standard precision (SP) docking.
Following preparation indexed libraries from both groups the samples were subjected to next generation sequencing.
Biopanned immuno-cDNA libraries were subjected to next generation sequencing, de novo assembly, and bioinformatic analysis.
Transcriptome and small RNA libraries were constructed and subjected to next generation sequencing.
72 out of 82 cases were subjected to next generation sequencing (NGS).
Following ChIP, the eluted DNA fragments were subjected to Next Generation Sequencing (NGS) using the Illumina Hiseq 2000 platform.
The MeDIP-seq approach employs an antibody against 5-methylcytosine or methyl-binding domain proteins to capture methylated DNA, which are subsequently subjected to next generation sequencing [ 11].
Both tumour tissue types, together with a blood sample, will then be subjected to next generation sequencing for a panel of cancer-related genes.
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