However, IFO is subject to limiting side-effects in high-dose protocols.
We used a single non-anesthesia observer to rate RSS in all study subjects to limit inter-observer variance.
Alternatively, cells were subjected to limiting dilution cloning.
Isolated nuclei from NIH-3T3 fibroblasts subjected to limiting digestion with MNase produce a ladder of DNA fragments corresponding to multiples of the nucleosome (Fig. 3, upper).
CIB1 over-expressing lines were established and subjected to limiting dilution, and 8 12 of the resulting subclones were cultured continuously for 6 weeks.
Diploid cells subjected to limiting nitrogen or haploid cells subjected to limiting glucose become elongated, exhibit polar budding, suppress budding in mother cells, remain attached after cytokinesis, and elaborate extracellular glucanases (Gancedo 2001; Palecek et al. 2002).
To isolate Fam20C-KO clones, U2OS cells were transfected with the TALEN plasmids by electroporation and subjected to limiting dilution.
To establish individual positive clones from single cells, the neomycin selected cells were further subjected to limiting dilution in 96-well plates for two-week colony selection.
While subject to limits, those deductions can bey large.
Investment banks should be subject to limits on leverage; hedge funds need scrutiny; and more.
