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Peter is hardly the only character subject to memory lapses.
TRAIL was then added at a final concentration of 500 ng/ml (Keogh et al., 2007; Agostinis et al., 2012) and the cells were subject to time-lapse microscopy, as previously described (Dash et al., 2003b).
After 48 h post-infection, ECs were subjected to time lapse imaging for 18 h (Figure 2C).
While Manuel was subjected to occasional lapses into casual 1970s xenophobia ("you dago dodo!"), the cultural rift between him and the Brits around him was full of comic potential – particularly his terrible language skills, a simple and sublime foil for Basil Fawlty's rising blood pressure.
For this approach, HeLa cell lines with an integrated GFP-tagged histone H2B were transfected with control or UNG2-directed siRNA, and subjected to time-lapse imaging.
THP-1 cells expressing α2AAR-YFP/CFP were labeled with the Vybrant DiD cell-labeling solution (DiD) and then subjected to time-lapse video microscopy.
Where indicated, DU145 cells-expressing EGFP-WOX1, EGFP-WOX1ww (WW domains only), or EGFP were treated with C1q (1 µg/ml) and subjected to time-lapse microscopy at 37°C and 5% CO2/atmosphere conditions for determining cell morphological changes, including cell shrinkage, membrane blebbing, and nuclei condensation, using a Nikon Eclipse TE-2000S inverted microscope.
The transfected cells were synchronized and subjected to time-lapse imaging.
Rat cortical neurons were subjected to time-lapse recording for 92 h after cells were infected with DR6 FL or control lentivirus.
(H ) MDA-MB-468 cells stably transduced with tet-shMELK and Histone 2B-GFP were cultured in the presence or absence of doxycycline for 3 days, and then subjected to time-lapse imaging.
1×10 cells were plated on 10 µg/ml fibronectin coated plates for three hours and then subjected to time lapse-video microscopy with a frame taken every 5 minutes for a total of 16 hrs.
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