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ΔCt was calculated for each subject specimen as the difference between Ct values of each target gene of interest and the GAPDH gene and averaged, and higher mean ΔCt values represent less gene expression.
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We subject specimens of β-phase Ti-6Al-4V to cyclic charging/discharging with hydrogen (by cyclic exposure of specimens to gaseous H2), under a small external tensile stress.
Human subject specimens were deidentified prior to use in this project.
On the basis of the rate of brain donation in recent previous work, we anticipate that 300 subject specimens will be donated over the 4-year study period, with 20%% acquired through the registry.
Tests were carried out by subjecting specimens to gravity and cyclic loads.
Construct strength was measured by subjecting specimens to ramp load until failure.
Moreover, this device can subject the specimen to µG conditions immediately, without exposure to the hypergravity and strong vibrations of a space launch.
Missions to Mars will subject living specimens to a range of low gravity environments.
In this subject only specimens from submandibular and sublingual glands were available (Figs. 9a,b).
There was less variability between subjects and specimen type in the all bacteria assay compared to the P. melaninogenica assay.
Before subjecting the specimens for FESEM analysis, all the specimens were prepared by placing them individually into an auto fine coater (JEOL Model JFC 1600, Tokyo, Japan).
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