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This should be studied as a remaining subject for confirmation of the study.
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Whole-genome high-resolution oligonucleotide microarray CGH analyses in patients D1 D9, for fine mapping of the sizes and extents of the deletions, and in subject D10, for confirmation of the deletion, were performed with the NimbleGen array HG18_WG_CGH_v1 with 385,000 or 2.1M oligonucleotides (NimbleGen Systems, Madison, WI, USA), in accordance with the manufacturer's instructions.
All of the PCR products were subjected to sequencing for confirmation.
Only samples with positive ELISA results were subjected to IFAT for confirmation of positivity.
At 16 weeks post-AOM, a group of 10 animals from each diet group and gender were randomly chosen and subjected to necropsy for confirmation of tumor presence.
Furthermore, the accessibility of patient's data from the KPNC electronic medical records will lessen subject burden and allow for confirmation of self-reported data.
Positive samples were removed from the machine and subjected to ZN staining for confirmation of the presence of mycobacteria, but no attempt was made to confirm the species.
PCR products of the virulence genes were subjected to restriction analysis for confirmation.
Positive cultures were subjected to AFB staining for confirmation of mycobacterial growth.
The resultant PCR product was sub-cloned into the pCRII-blunt-TOPO vector (Invitrogen) and subjected to DNA sequencing for confirmation.
A subset of small tumors, those imageable by fluorescence and/or ultrasound but not apparent to the naked eye when looking at the intact pancreas, were subjected to histologic examination for confirmation of imaging findings.
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