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Reciprocal blast searches using H99 chromosomes as queries and JEC21 as the subject database were used to confirm the search results.
To identify the rearrangements, we first did nucleotide blast searches (blastn) using individual chromosomes of JEC21 as separate queries and the complete H99 genome as subject database.
After the one-to-one correspondence of chromosomes was established between the two genomes, we performed all-against-all blast searches (blastn), each time using one chromosome from JEC21 as a query and its corresponding chromosome from H99 as the subject database.
The final subject database comprised 52,355 expressed sequences.
The genomes of the Methanomassiliicoccales representatives were not included in the subject database.
Study participants were recruited from a research subject database and by newspaper advertisements.
Similar(43)
The subject databases used were InSpec, ByggDok and the database ScienceDirect.
Articles are assigned to the different databases according to the subject rather than the journal they are published in, so that articles from any one journal might appear in all three subject databases.
It is used, for example, by the biosoftware_bench software server to allow users to upload datasets for use as query or subject databases.
The present study was conducted by cross-linking Medications Prescriptions Database with Health-assisted Subjects' Database, Hospital Discharge Database, and Mortality Database.
There were more than 28.7 million potentially eligible subjects in the study database.
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