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Finally, the vasodepressor responses induced by electrical stimulation or exogenous α-CGRP in the different subgroups of animals were compared with a two-way analysis of variance.
To evaluate cell proliferation, subgroups of animals (n = 12) were pulse-dosed with 50 mg/kg (0.5 ml ip) bromodeoxyuridine (BrdU) 4 hours prior to euthanasia.
Subgroups of animals were weighed and euthanized (Nembutal, 60 mg/kg ip and pneumothorax) at 1, 3, and 7 days after treatment for endpoint analysis as described in the sections below.
In some experiments, entire subgroups of animals [Cheney & Seyfarth, 1980] have been filmed.
At the end of the 3 weeks, subgroups of animals were sacrificed at 3 h intervals over a complete 24 h cycle, starting at 08 00 h.
Compared to pedigree-based relationships, all estimated genomic relationships will be biased upwards, but bias will be the same across breeds and subgroups of animals.
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It is noted that the two subgroups of animal Trithorax proteins differ by the position of the FYRN- and FYRC-domains (juxtaposed or separated), while the plant Trithorax homologues, ATX3, ATX4, and ATX5, do not carry DAST motifs [ 31].
To test the sustainability of the behavioral improvement, GDNF-secreting capsules were withdrawn in a subgroup of animals, 7 weeks post-implantation.
In a subgroup of animals with the worst hemodynamic impairment, EA in the control and PMX-HP groups was 0.50 ± 0.29 and 0.29 ± 0.14, respectively (p = 0.018).
The difference between the changes in diastolic blood pressure within one subgroup of animals was evaluated with Student-Newman-Keul's test, once a two-way repeated measures analysis of variance revealed that the samples represented different populations [20].
The difference in the absolute values of diastolic blood pressure and heart rate within one subgroup of animals before and during the continuous infusions of methoxamine (20 μg/kg·min) and DHE (3.1 μg/kg·min) were evaluated with paired Student's t-test.
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