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The one major subdivision of patients we could perform analysis on was trauma versus non-trauma patients.
Subdivision of patients into risk groups using the unsupervised clustering-based approach described above allows assessment of the predictive power of a gene signature, but does not allow specificity (fraction of low-risk patients correctly classified) and sensitivity (fraction of high-risk patients correctly classified) to be tuned according to specific requirements.
The combination of both mucosal MMP-based parameters appeared as a highly significant discriminator (LR 20.30, P<0.0001) for subdivision of patients into good, intermediate and poor survivors as shown in Figure 1D.
However, subdivision of patients according to their NPM1 and FLT3-ITD mutational status revealed significantly lower BAALC expression in the group of patients with NPM1mut and FLT3wt or FLT3-ITD/ FLT3wt<0.5 expression compared to patients with FLT3-ITD/ FLT3wt⩾0.5, irrespective of their NPM1 mutational status (39.4 vs 374.8, P<0.001).
Microarray analysis of RA synovial tissues has revealed a noteworthy subdivision of patients, with one group having abundant expression of genes involved in adaptive immune response and another group expressing stromal cell-related gene clusters with a tissue remodeling signature [ 14].
The study population included 4 subgroups, all recruited from among inpatients at the University Hospital "Policlinico di Monserrato" of Cagliari; stratification by age implied a subdivision of patients of each subgroup into four age groups: 18-24, 25-44, 45-64 and > 65 years (Table 1).
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The genetic characteristics would be helpful for the further identification of the clinical subdivision of SMA patients.
We, therefore, thought that subdivision of these patients based on lymph node metastasis and number of risk factors was warranted.
After subdivision of the patients according to increasing SACE levels: less than 16.0 (mean SACE of lung cancer--s.d)., 16.0-22.0, 22.1-28.2 and greater than 28.2 nmol/ml/min (mean SACE of lung cancer + s.d).
Further subdivision of the patient cohort into subgroups with regard to individual patient treatment or additional clinical parameters was not considered in the study presented here but should be investigated in a larger patient cohort to examine the predictive value of HER1 to HER4.
Very likely, a careful subdivision of this heterogeneous patient group will be indispensable to obtain statistically significant results.
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