Sentence examples for subcultured in from inspiring English sources

Cells were further subcultured in McCoy-5A (Lonza, USA) medium supplemented with ascorbic acid and L-glutamine (Lonza, USA).

In all growth assays, bacteria were subcultured in 50 ml of growth media for a starting OD600 of 0.05.

Canine anterior cruciate ligament (ACL) cells were isolated from the anterior cruciate ligament and subcultured in DMEM containing 10% FBS.

Bovine keratocytes were subcultured in DMEM/F-12 containing 10% fetal bovine serum (FBS) through passage 5.

The sample recently isolated from animals (Pb18b) demonstrated a greater capacity to adhere and to invade the Vero cells than the one subcultured in vitro (Pb18a).

Bacteria were subcultured in fresh BHI broth to log phase growth, as measured by optical density, pelleted washed, and resuspended in DMEM for in vitro experiments or isotonic saline for in vivo experiments.

These neural rosette ipNPCs were then individualized, and subcultured in NSM on Matrigel.

8TF-induced Best1::GFP+ colonies from HFF were picked and subcultured in RA plus SHH condition.

The mean methylation level of regenerants showed a 0.28% to 3.95% decrease in seedlings subcultured in vitro for nine generations.

Furthermore, we studied the methylation level of the regenerants from the ninth subcultured in vitro seedlings to investigate the effect of methylation level on somaclonal variations.

At 75% confluence, the cells were harvested using 0.25% trypsin and subcultured in 75-cm2 flasks, 6-well plates, or 96-well plates.