Sentence examples for subcultured from from inspiring English sources

Then, all isolates were subcultured from a single colony to acquire a pure colony.

Being transferred to our laboratory, isolates were streaked out on the plates of Columbia agar (Oxoid Ltd., UK) supplied with a 5% of sheep blood, to form isolated single colonies; pure cultures were subcultured from single colonies after the overnight incubation at 37 °C in air with 5% CO2.

Multiple isolates were subcultured from one or both of the two different selective agar plates for each sample.

Non-piliated OpaB-expressing bacteria were subcultured from the freezer and passed once to GC agar before being suspended in McCoy's 5A medium supplemented with 2.2 g/L sodium bicarbonate and 5 mg/L Fe(NO3 3 to an A600 of 0.07.

H. pylori was subcultured from the stock.

Isolates of potential interest were subcultured from spores obtained directly from newly formed fruiting bodies.

We recently observed that transgenic plants of torenia grown from explants subcultured for several generations are all male sterile.

GFP-expressing hairy roots (~2 cm) were excised from cotyledons and subcultured for 4 d on OMS medium containing 400 mg l-1 Timentin.

To further confirm the functionality of Wnt3a expanded hPDLCs, we subcultured hPDLCs from passage 4 with or without Wnt3a up to passage 9 and compared the functionalities of Wnt3a-pretreated and control cells.

Prior to each assay, bacteria were subcultured twice from frozen stock preparations onto TSB supplemented with 1.2% (wt/vol) agar plates and incubated aerobically at 37°C for 24 48 h.

Zaid (1981), working with date palm leaf explants from adult trees, offshoots, seedlings and asexual plantlets, found that only subcultured leaf callus from seedling and asexual plantlets produced roots.