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Both the HSA- and rCTB-specific responses were followed quantitatively (as assessed by the anti-[IgM + IgG] level, the s-IgA response and the delayed-type hypersensitivity [DTH] response), or qualitatively (by the IgG subclass profile).
Whereas analysis of the serum IgG subclass profile indicated a strong IgG2a response and thus a systemic Th1-type pattern, this vector also induced a good mucosal IgA response.
Human IgG subclasses were measured using human IgG Subclass Profile ELISA kits (InVitrogen).
The serum level of each IgG subclass was determined using the human IgG subclass profile ELISA kit (Invitrogen, Carlsbad, CA) based on a sandwich-type ELISA method.
This specific subclass profile is a very consistent observation in all three models and has been associated with the most potent proinflammatory and effective antibody response.
Figure 5 shows the IgG subclass profile for KMP-11 recombinant antigen.
Similar(46)
These 2 forms of MGN may have different pathogenic mechanisms, with different IgG subclass profiles in the immune deposits.
The conjugates retained potent adjuvant activity, low toxicity, and improved activity toxicity profiles relative to QS-21 itself and induced IgG subclass profiles similar to those of QS-21, indicative of both Th1 cellular and Th2 humoral immune responses.
This investigation was designed to explore the polymorphisms at FcγRIIa gene in association with both anti-malarial total IgG antibody and IgG subclass profiles to C-terminal regiof of Plasmodium falciparum merozoite surface protein 1 (PfMSP-119).
The analysis of dispersed cells revealed a higher frequency of cells secreting IgG than IgA and the predominant Ig subclass profiles were IgG1 > IgG3 and IgA1 > IgA2, respectively.
The study of IgG subclass profiles between different vaccine regimens used in clinical trials with divergent efficacy outcomes can provide information on the quality of the vaccine-induced B cell response.
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