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For IgG subclass determination, 100 µL of HRP-rabbit anti-mouse IgG1 (AbD Serotec, Kidlington, UK, 1∶5,000) or HRP-rabbit anti-mouse IgG2a (AbD Serotec, Kidlington, UK, 1∶2,000) was added.
IgG subclass determination indicated a predominant Th2-type immune response similar to that observed in BALB/c mice vaccinated with a UV-inactivated SARS-CoV plus alum (7).
IgG1, IgG2 and IgA subclass determination was done as described earlier [ 39] by individually combining constant region-specific primers for IgG1, IgG2 and IgA genes (CG1d, CG2a and CA1, respectively) with a set of variable heavy chain primers: VH135, VH3a, VH3f, VH4, VH4b.
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Microarray use in pharmaceutical research has expanded with applications in basic research for drug target discovery, biomarker determination and validation, and toxicogenomics as well as development of prognostic tests and disease-subclass determinations.
Lipid profile measurements and lipoprotein subclass particle concentration determination were performed as previously described [ 30].
On day 42, eye bleeds were taken by orbital puncture for IgG subclass and HI titer determinations.
In that study, it was demonstrated that determination of IgG1 subclass antibodies to chlamydial HSP60 increased the diagnostic value of the ELISA in identifying TFI.
We summarized the overall composition of FA chains in the dPtdEtn subclass of bovine heart PtdEtn without determination of their regiospecific positions (Figure 7B).
As shown in Table 1, Bacteria have two SMR homologues per bacterium based on the overall average but the determination of which SMR subclass member participates in this average is difficult to identify since SMR sequences distributed more or less equally within all three SMR subclasses.
NMR analysis is precise for the determination and quantification of lipoprotein subclass [93].
However, previous investigations of the humoral immune response to HSP60 in SpA patients have lacked determination of immunoglobulin G (IgG) subclasses and patient follow-up.
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