IFN-alpha boosted mice showed a very similar antipeptide glycolipid antibody levels of IgG, IgM, and IgA as well as all IgG subclasses analyzed (Table 1), and/or cytokine production (Table 2) compared to BCG-vaccinated/PBS-boosted mice, or control mice (Tables 1 and 2).
Immunoglobulins and IgG subclasses were analyzed by nephelometry (DADE Behrings BNll); subclasses were determined using a commercially available kit (The Binding Site Group Ltd., Birmingham, UK) and the results are expressed in g/L.
To simplify the analysis, TIR and non-TIR subclasses were analyzed separately.
Lipoprotein subclasses were analyzed using the VAP-cholesterol technique, precluding extrapolation to results based on other analytical techniques that quantify numbers or size of particles or associated protein.
To further substantiate the difference between the type of cellular responses elicited by the whole viral pandemic H1N1 California and H5N1 Vietnam vaccines in comparison to the split seasonal H1N1 Brisbane vaccine, anti-HA IgG subclass responses were analyzed by ELISA as described in Methods, using sera collected on day 42.
Within the subclass Palaeognathae we analyzed the white-throated tinamou (Tinamus guttatus), the African ostrich (Struthio camelus) and the emu (Dromaius novaehollandiae).
A summary of both analyses are shown in Figures 2, 3, 4, where a distinct pattern of residue conservation can be observed for each SMR subclass member we analyzed.
With regards to the eight subclasses of DUBs analyzed, the Josephins (MJD), UCHs (peptidase_C12), autophagins (peptidase_C54) and deSUMOylases (peptidase_C48) families are well conserved within and between species with the exception of a clear differential expansion in the A. thaliana UCHs and deSUMOylases.
We analyzed LDL subclass in type 2 diabetics and normal controls with LipoPrint LDL System to investigate the LDL heterogeneity in diabetics and factors affecting it.
Isotype- and subclass-specific IAs were analyzed as described previously (12).
