Subcellular localization experiment showed that both IiPALs were localized in the cytosol.
This postulation is supported by the OsRUS1 subcellular localization experiment using a rice protoplast transient-expression approach.
To test whether H2O2 promotes nuclear accumulation of SIRT1, we performed a subcellular fractionation experiment.
Subcellular fractionation experiment was performed as previously described (Buchkovich et al., 2013).
Combining bioinformatics prediction with subcellular localization experiment analysis, AsCOI1 was appeared to locate in nucleus.
Nevertheless, data from the subcellular localization experiment showed that Sin1 is excluded from the nucleus when transfected in COS1 or L929 cells [ 4], suggesting these NLS are probably not functional.
To determine if vezatin is a cytoplasmic or a plasma membrane protein (either integral or membrane-associated), we carried out whole-cell protein extraction with or without Triton X-100, and a subcellular fractionation experiment by means of density gradient centrifugation.
Indeed, in the subcellular fractionation experiment we found that the quantity of isolated mitochondria was at least 2-fold higher in cells from 4.5 M NaCl, and that the succinate dehydrogenase activity per μg of mitochondria was 2.5 ± 0.5-fold higher compared with mitochondria from 3 M NaCl (data not shown).
The ASL3 protein was predicted to contain a chloroplast transit peptide (cTP) in its N-terminal region, suggesting that the protein is one of the PPRs targeted to chloroplast, and subcellular localization experiments confirmed this prediction.
Subcellular fractionation experiments revealed that the fraction of Pb in the form of MRG was much lower for the mussel, confirming, as observed for other metals, that MRG-associated Pb is not available for trophic transfer to decapod crustaceans.
