Sentence examples for subcellular detection from inspiring English sources

Since AtAPY1 was not detected in the cell wall by three subcellular detection methods and did not exhibit the substrate specificity of an ecto-apyrase, we hypothesize that some apyrase other than AtAPY1 is the regulatory enzyme observed in eATP signaling of Arabidopsis.

A unique feature of APEX2 is that it retains robust peroxidase activity even after strong fixation with 2% glutaraldehyde and brings about its utility as a tag for subcellular detection of proteins of interest with electron microscopy (EM) with clarity not attainable by the more conventional immuno-electron microscope technique (Rhee et al., 2013; Lam et al., 2015; Lee et al., 2016).

For the subcellular detection of β-catenin by immunofluorescence, cells were fixed in 4% paraformaldehyde for 30 minutes.

The subcellular detection of flavonoids was achieved after staining with Wilson's Reagent.

The RNAscope method thus allows clear subcellular detection of RNA spots within the cytoplasm and the nuclei of zebrafish embryos (see Figure  2I and Additional file 7 for myoD RNA).

Moreover, because CFP and YFP fusions generally retain activity and maintain their normal subcellular localizations, the detection of CFP YFP FRET in live cells typically reflects the steady-state association of functional proteins in their native states.

Following stimulation, cells were processed for nitrogen cavitation, subcellular fractionation, and detection of proteins of interest by western immunoblotting.

The discovery and characterization of protein movement is technically challenging, given the necessity for sensitive detection and subcellular resolution.

The analysis of single cells or subcellular particles by the detection of their light absorption, scattering and/or fluorescence properties as they pass through a laser beam in a directed fluid stream.

For example, the use of U-shape kinked nanowire structures would yield ultrasmall nanoFET detectors with a very high aspect ratio26, and thus enhance capabilities for specific targeting, multiplexed experiments and deep tissue/cell insertion and detection with subcellular resolution.

The design of robust tools for hypochlorous acid (HClO) detection at subcellular level is highly desired for the better understanding of its biological function in cellular signaling pathways.