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They comprise sub-set 2. These genes contained a 12 bp motif in their upstream regions, also seen at varying positions from the translational start.
Three genes peaking at 2 h post-infection were classified into sub-set 1.
The genes that peaked at 6 h- 12 h post-infection were included in sub-set 1.
Sub-set 1 contained three enzymes involved in the transport and modification of purines (AT, IMPDH and GMPS), and also one pyrimidine-modifying enzyme (CTPS).
Based on their real-time PCR expression profiles, the genes were divided into 3 sub-sets (sub-set 1, 2 and 3).
The best feature selection strategy achieved a slightly better performance than the manually selected GO sub-set (1 sd unit or more), but still inferior to the 20 curated neurally-relevant features (Table 3).
However, our analysis using mtDNA data (data set c24p and 23 sub-data sets) provides moderate to strong support for intraclass relationships in the Rhodymeniophycidae.
Out if this sub-set, 421 respondents were PMTCT testers.
Sub-set 3 consists of 3 genes with expression levels peaking at 72 h post-infection.
Most of the MCM-like proteins peaked at 48 h post-infection and were classified into sub-set 3.
These were classified into sub-set 2. The most significant motif found specific to this sub-set was an 11 bp motif occurring one or two times in the upstream regions of this sub-set.
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