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He is a student from Tripoli, studying translation at university in Cairo.
By studying translation initiation, we hope to gain insight into potential error-correcting aspects of genomic sequences and systems.
In the course of studying translation termination accuracy at the stop codon of the essential IMP3 gene of S. cerevisiae, we constructed a mutant allele of IMP3 where the endogenous TAA stop codon was changed to a glutamine codon (CAA).
Studying translation initiation in these organisms may not only help define divergent features in specific stages, useful in the identification of potential therapeutical targets, but may also enhance the understanding regarding the evolution and conservation of the whole process throughout the eukaryotic lineages.
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The purpose of the work is to study translation's peculiarities of ethnic cultures' meanings and their connection with the specifics of shaping.
Here, we develop a whole-cell model of protein translation, and we apply it to study translation dynamics in yeast.
To study translation of GCN4 main ORF, Grant et al. [ 4] further made different pairs of GCN4-lacZ constructs as shown in Figure 1D and 1E.
In order to study translation and, more specifically, stop codon readthrough in D. melanogaster, we sought to develop a robust ribosome profiling assay for this organism.
The protocol has been used to map RBS in embryonic stem cells [ 27], to study the effect of drugs in cancer therapy [ 28], for mapping of the RBS in yeast [ 25] and to study translation dynamics in bacteria [ 29].
The last 10 years have witnessed an explosion of high-throughput technologies that are changing the way we study translation, are revealing unexpected layers of complexity and are providing a new dimension to the translational control field.
To study translation, cells were metabolically labeled with S-methionine (20 μCi/ml) (Perkin-Elmer, Waltham, MA, USA) in DMEM lacking methionine (MP Biomedicals, Santa Ana, CA, USA) supplemented with 0.1% FCS for 2 h at 37°C (pulse).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com