The aim of this study is to construct, obtain and study expression in eukaryotic cells of recombinant plasmid – candidate DNA-vaccines inducing T-cell immune response against breast cancer.
We used immunohistochemistry to study expression in developing mouse lung of calcitonin gene-related peptide (CGRP), Clara cell 10-KD protein (CC10), and surfactant protein-A (SP-A), markers that are differentially expressed in neuroendocrine cells, Clara cells, and Type II alveolar cells.
One can begin by using transgenics to study expression in vivo.
Most of these have studied expression in either blood or brain tissue samples.
Longerich et al (2011) demonstrated Annexin-A2 expression in HCC in a small western European patient cohort, but did not study expression in TFL tissue.
We initially studied expression in a panel of established breast carcinoma cell lines and demonstrated that expression was reduced in several of the cell lines.
Since it was not possible to study expression in all accessions, we chose the above described "cold core" for detailed analysis.
To study expression of dysadherin in human testis, epididymis, and spermatozoa.
g qRT-PCR analysis to study expression of OsMKK6 in OsMKK6 EE overexpression lines T3 generation and control plants.
In order to understand more about the role of EN2 in cancer, we began by studying its expression in cells lines derived from tumors and normal tissue.
