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The use of hair colorants in the twin study was ascertained via a questionnaire which first asked if subjects used hair colorants and then asked how long ago they had last used hair colorants (see SI Methods for further details).
Patient eligibility for participation in the study was ascertained by doctor completion of a study-specific case report form (CRF).
The vital status of all EPIC-Norfolk participants for this study was ascertained up to 31 March 2010 through linkage with the UK Office for National Statistics.
Specificity of the antibodies used in this study was ascertained by nuclear immunostainings and by the appropriate size of a single-reactive band to the predicted molecular weight.
Sequence orientation of approximately 78% of the unigenes discovered in this study was ascertained, along with the annotation of their function by searching against public databases.
The primary dependent variable used in our study was ascertained through the following question to the parent or guardian: "Has a doctor or health professional ever told you that selected child (S.C).
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The families used in this study were ascertained through the Kathleen Cuningham Foundation Consortium for Research into Familial Breast Cancer (kConFab).
Subjects in this study were ascertained as part of the Grady Trauma Project, which is an ongoing molecular genetic study with a primary focus on posttraumatic stress disorder.
Thus exposure data in this study were ascertained on all cases of cerebral palsy and a subset of all preterm infants enrolled in the longterm follow-up program.
The 107 additional CRA cases in our study were ascertained after recruitment to UKCAP was completed, and were therefore not considered for entry into the UKCAP study, thus, aspirin or NSAID use was not an exclusion criteria for these participants.
Dietary habits during the 6 months preceding enrolment in the study were ascertained through a validated food-frequency questionnaire covering frequency and quantity of consumption of about eighty food items and beverages [ 12].
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