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In addition, studying the splicing of introns with weak splice sites may facilitate the identification of splicing factors that are important for splicing in vivo, but may be dispensable for detection of basal splicing of introns with strong consensus splice sites, which have traditionally been used in most mechanistic studies of splicing in vitro.
Furthermore, our detailed study of the splicing outcome of this acceptor-splice site mutation adds to the growing number of studies investigating complex splicing outcomes and determining additional factors needed for correct pre-mRNA splicing.
We study the Memory Arrangement (MA) Problem.
Approximately 74% of soybean genes exhibited pattern h, which has a highly conserved splicing arrangement of three exons and two introns in the R2R3-MYB domain.
We also define a measure of the conservation of the arrangement of exonic splicing regulators and study the variation of this arrangement and the relation to the sequence divergence of exons and introns.
Enzweiler studied the arrangement of the bricks.
Further, we have also defined a measure of the variation of the arrangement of exonic splicing enhancers (ESE-conservation score) to study the evolution of splicing regulatory sequences.
Three columns were provided with short lap-splice length of longitudinal reinforcements at the plastic hinge location to study the effect of lap splice.
The magnitude of the alterations in the splicing process can be studied through the splicing index.
To study the regulation of these alternative 5' splice sites, we made two mutants which improve the match of the large-T-antigen 5' splice site to the 5' splice site consensus sequence.
The diversity of the splicing step has been intensively studied.
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