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To our knowledge, this is the first genome-wide study of transcript profiles across zygotic embryogenesis in pines.
To our knowledge, this is the first genome-wide study of transcript profiles in C. sativa seeds at different developmental stages.
The most comprehensive study of transcript profiling in Pinus embryos has been conducted in P. taeda, where approximately 68,700 ESTs have been generated from somatic and zygotic embryos [ 9].
A study of transcript abundance in response to wounding, dehydration, and to hormonal treatments, ethylene and jasmonate, helped to determine what types of abiotic stress and signalling pathways occurred during tapping.
In their study of transcript variation across eukaryotes, McGuire et al. [ 26] showed that alternative splicing in Cryptococcus is dominated by intron-retention events (in which the entire sequence that is spliced out of one transcript of a gene is entirely retained in another transcript).
To understand the regulation of the different XTH genes identified through the transcriptome sequencing, a time course study of transcript accumulation of various rose XTH genes was carried out by real-time RT PCR in 0.5 µL L−1 ethylene-treated petal AZs of R. bourboniana and R. hybrida as well as under conditions of natural abscission.
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The heterologous hybridization microarray was successfully applied in the study of transcripts changes associated to papaya fruit, a commercially relevant crop from a non-model organism.
Recent studies of transcript profiling in pollen indicate even higher percentages of pollen-specific gene expression [3], although the vast majority of genes expressed by microgametophytes still appear to be expressed during both the sporophytic and gametophytic stages of the life cycle.
The samples were frozen in liquid nitrogen and stored at −70°C for the studies of transcript expression.
Initial studies of transcript expression used the expectation maximization (EM) approach (Li et al., 2010; Nicolae et al., 2010).
Time course studies of transcript levels upon stress application together with the study of promoter expression in vitro and in planta are currently being undertaken to elucidate the regulatory mechanisms underlying the observed differences in transcript abundance.
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