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In addition, identifying targets by altering miRNA expression and measuring changes in mRNA levels returns no information on which targets might be the most important in carrying out the actual biological processes (e.g. cellular differentiation) and is limited to the study of the altered miRNA.
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In our study, most of the altered bands do not have any homology with any other bands in the same RAPD profile as confirmed by Southern hybridization of the altered bands to the RAPD profile.
To study the consequences of the altered phosphorylation state of Lck and Fyn in LAT-depleted cells, we analyzed the phosphorylation of direct substrates [4], [5].
Several rodent models are currently available to study the effects of the altered HD gene.
In order to study the consequences of the altered chemokine homeostasis in Alox15 -/- mice, we investigated CXCR2 expression on neutrophils.
The results of this small, preliminary study require verifying in larger studies, and the significance of the altered hepcidin levels in the obese are currently unclear, complicating interpretation.
This may also be the case in the elderly, but studies investigating the metabolic consequences of the altered elderly microbiota are required.
Our studies do not define the mechanism of the altered testosterone/estrogen ratio.
There are several reports studying the altered properties of erythrocytes during the senescence process [2], [3], [27].
They exposed the mold to X rays and studied the altered nutritional requirements of the mutants thus produced.
Furthermore, this is an important issue in the study of cancer, since altered intestinal absorption may alter blood chemistry and contribute to anorexia genesis.
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