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The variation of the study locus may not be well captured, the true effect of the particular polymorphism may be small and thus, may require a large sample size to be detected.
From our study, locus CAGC-235 showed parallel allelic trends in more than two lake systems.
These include novel transcripts identified in this study (Locus tag labelled as "NA").
We used 16 microsatellites from our multiplex (having excluded the study locus DYS385 and the other duplicated marker YCAII) under a model of constant population size.
> -wrap-foot> In the present study, locus clusters were defined as regions in which ≥10 loci were mapped in 5 cM windows.
To further study locus drop out and error rates, we examined the effect of error rate on the distribution of errors per read.
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In this study, loci duplication, rearrangement and translocation were all detected by the analysis of duplicated loci.
This resulted in relatively high coverage numbers for the study loci; although coverage numbers were variable, all samples had greater than 100× average coverage (Table 1).
In a follow-up study, loci regulating bleomycin-induced lung fibrosis were mapped in fibrosis-prone C57BL/6J mice compared with the fibrosis-resistant A/J strain [ 31].
As control, we tested the dependency of methylation at CpGs within one studied locus (i.e.: LINE-1 SN-8 and SN-9; Alu SN-1 and SN-4).
TCF7L2 is the most intensively studied locus for T2D risk so far.
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