Additional limitations of this study include limited availability of clinical data in the RA cohort used for the initial gene-signature discovery owing to the retrospective nature of interrogation of clinical chart data after sample collection from joint surgery, and a lack of consent for chart review in some cases.
The review however had several limitations including differences in zinc doses, sample size, study duration, limited availability of data on zinc intake and variation in baseline parameters and so the conclusions remain in doubt.
15 The previous studies investigating the association between NSAIDs and AF are mainly retrospective case control studies or claims database studies with limited availability of potential confounders.
Due to the retrospective nature of the present study, the limited availability of specialists, and the costs of performing advanced diagnostic imaging and further investigations, the vast majority of dogs have not undergone a complete diagnostic evaluation.
The major drawback to promptly accessing ACTs found in this study was the limited availability of subsidized ACT in places where most caretakers took their children for care.
However, other heat shock proteins not examined in this study (due to limited availability of serum samples) such as HSP90 or HSP72 may have been responsible for this protective effect.
Challenges presented included the requirement of significant financial resources for development of study materials and limited availability of validated tools to measure health care professional collaboration over time.
The scale of such studies, and the limited availability of both inoculum and recipient sheep precludes a study design which includes every genotype/strain/route of challenge combination.
The conventional Ussing chamber has been of limited use for human studies because of limited availability of tissue samples of adequate size, i.e. surgical specimens.
Within the Lophiiformes, interrelationships among 18 families and five suborders have been inadequately studied, owing to limited availability of specimens from the most taxonomically rich suborder Ceratioidei.
