There will be no adjustment for multiple comparisons as all comparisons have been determined a priori and, while adjustment maintains study wise error, it may preclude detection of clinically important differences [ 36].
Doing this while it is not yet possible to fully resolve the inflation of false identification resulting from combining independent studies, the results will be reported study wise so the user can assess the reliability of the results by checking other supportive information from each study.
You will need these items and it is highly recommended as it is not only good study wise, but also good for your health.
A study-wise statistical significance threshold of 3.9×10−7 was used to correct for the number of tests (10 risk factors ×12,808 gene expressions).
In this report we found that two allelic variants of the PRKCI gene were associated at a study-wise significant level with a decreased risk of prostate cancer.
Potential binding sites of transcription factors within the sequence encompassing the two study-wise significantly associated SNPs were performed with MatInspector Professional (http://genomatix.de/cgi-bin/matinspector_prof/mat_fam.pl) [54].
At study-wise significance, the number of cis and trans eQTLs were 2,477 and 349, respectively, yielding a cis/trans ratio of 7.1 (81 eQTLs were both cis- and trans-modulated).
To investigate gene expression in relation to risk factors (age, gender, BMI, HDL and LDL cholesterol, triglycerides, Systolic and Diastolic Blood pressure, smoking and plasma CRP), the study-wise significance threshold was set at 3.9×10−7 to correct for the number of risk factors (n = 10) and expressions (n = 12,808) tested.
SNPs with a study-wise MAF <0.05 were included in the analysis.
The study-wise Meff obtained was 31 and the adjusted threshold for significance was 0.05/(31) = 0.0016.
