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Cells for immunolabelling studies were seeded at a concentration of 2 x 10 cells/mL into Falcon 6-well tissue culture dishes containing 18 mm glass coverslips or 75 cm tissue culture flasks containing 40 mL of RPMI 1640 supplemented with 10% FBS in the absence of antibiotics.
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The EGSB reactors in the present study were seeded with anaerobic flocculent sludge from the local POME treatment plant.
Briefly, the cells under study were seeded, supernatant samples were collected three times per day (6 h intervals) and absorbance was measured at 510 nm using the NovoStar MBG Labtech microplate instrument.
In contrast, the populations in this study were seeded with a modest amount of genetic variation and exhibited a mixed mating system, similar to wild populations of C. elegans.
This study was seeded at a rate of 320 pure live seed (PLS) per meter square.
In LOF studies, cells were seeded to 30% confluency before siRNAs were transfected using Lipofectamine RNAimax (invitrogen) at a concentration of 20 µM.
In these studies, cells were seeded by flooding cell suspensions on the arrays and letting cells fall randomly into the microwells.
For single-agent studies, cells were seeded and allowed to settle for 24 h before treatment with increasing concentrations of drug and incubate it for further 72 h with 5% CO2 at 37°C.
For differentiation studies, cells were seeded at confluence on the film surface.
For all IF studies, cells were seeded on cover glasses and grown for 2 days.
For viability studies, cells were seeded at 2500 cells per well in a 96-well plate.
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