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The lipid raft fractions in current studies were isolated from whole type II cell lysate and thus contained mixed lipid rafts from all of the subcellular compartments.
However, this second pathway, which requires a functional telomerase [27], [58], was not observed in our studies probably because most of the dicentric GCRs characterized in our structural studies were isolated in tlc1 mutant strains.
The fruiting bodies and mycelia of G. vittiformis after the bioaccumulation studies were isolated and washed with distilled water and oven-dried at 60°C (Rotek, India).
Since the method requires methylated parental plasmid DNA, all plasmids used in these studies were isolated from XL1-Blue cells (Stratagene) that have a dam+ (wild type) genotype encoding Dam methylase.
In a literature review of MEDLINE (1966 December 2013), EMBASE (1980 December 2013), ISI web of knowledge (v4.2) and Cochrane (1993 December 2013) databases combining the terms: 'proteomics', 'proteomic', 'preeclampsia', and 'PCOS' or 'polycystic ovary syndrome', no studies were isolated, where proteomic biomarkers for PE had been specifically investigated in women with PCOS.
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RNA from LCM studies was isolated using TRIzol and 4 µg glycogen (Ambion, Austin, TX) as a RNA carrier.
The fungi used in this study were isolated from an agricultural soil of the INPV.
The fungi selected for this study were isolated from the host plants belonging to genus Erica.
All the six triterpenoic acids, used as standards in the study, were isolated from the gum resin of Boswellia serrata.
The lipolytic, proteolytic and bacteriocin-producing LAB strains used in the study were isolated from the homogenised freshwater fish viscera.
All strains used in this study were isolated from those fruit bodies which were later used for extraction and bioactivity testing.
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