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Tissues for ultrastructural studies were fixed in 2% glutaraldehyde, post-fixed in 1% osmium tetroxide, dehydrated in graded ethanols, and embedded in Epon 618.
Subcutaneous tumor size was calculated by using the formula: {text{Tumor}};{text{volume}}; = ; {text{length}}; times ; {text{width}}^{ 2} ; times ;0. 5 2. Tumor samples and the normal organs from therapy studies were fixed immediately with 4%% paraformaldehyde.
Tumors and tissues collected from our animal studies were fixed in 10% neutral buffered formalin.
Tissues for histological and immunohistochemical studies were fixed in 10% neutral-buffered formalin and embedded in paraffin.
Hepatic tissues harvested from animal experiments or cells from the in vitro studies were fixed in RNAlate solution (Ambion, Inc., Austin, TX, USA).
One part of the samples which would be used for RT-PCR reaction and Western-blot detection was placed in freezing tubes and preserved within 15 minutes when they were placed in a −80°C refrigerator, and the other parts of the samples which would be used for immunohistochemical studies were fixed with 4% paraformaldehyde and embedded in paraffin.
Similar(54)
It utilizes constrained molecular dynamics where atoms determined to be immobile from difference distance matrix studies are fixed.
The main techniques used in these studies are fixed point techniques, Leray-Schauder theory, or upper and lower solutions methods (see, for example, [10 12] and the above references).
Tissue used for immunohistochemical and immunofluorescence studies was fixed in either ethanol (70%) or 10% neutral buffered formalin overnight and then processed for pffin embedding and 6 μm thick sections were generated as described [ 45] using a Leitz 1512 microtome.
Egg envelopes from ova fixed in formalin appeared opaque, and removal of the egg envelope was necessary to view developmental characteristics, although only two samples in the present study were fixed in formalin.
Samples used in this study were fixed in formalin within 20 minutes of removal.
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