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The studies were established using GCMS results and multivariate data analysis.
However, these studies were established using a murine model, and since it was later shown that mice have no S100A12 in their genome [ 20], alternate receptors are implicated [ 25].
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Contrary to earlier undertakings of the challenge the dose response curves in our study were established using a method that accounts for the individual cell lines' growth rate and AUC 0 was used as summary statistic.
Carbohydrate fermentation profiles of the strains in this study were established using API 50 CH (Biomérieux) according to the instructions of the supplier, with readout after 48 hr of incubation at 42°C.
The study is established using causal research design in order to examine the cause-effect relationship between the study variables.
Trustworthiness of the study was established using within-project evaluation and was accomplished by multiple methods of data collection (interviews and document analysis), iterative data analysis and coding, member checks of themes with several participants.
The diagnosis of OP in this study was established using WHO criteria,1 so that osteoporosis was defined as a T-score of less than -2.5 SD.
Of note was that both the transgenic animal model [ 21] and the FOP patients [ 23] have genetic mutations while the animal model that we used in this study was established using biological agents after birth.
The animal resource used in this study was established using breeds that diverged with respect to obesity traits in the parental generation, resulting in a F2 population that was highly divergent with respect to obesity and obesity-related traits.
Prescription profile of all FPs/GPs from the Quebec City area, including all physicians participating in the study, is established using the Régie de l'Assurance Maladie du Québec (RAMQ) databases.
In this research, based on the precipitation data in the last 40 years and topographical maps at 25 observation stations in and around Dingxi County, Gansu Province, China, the raster digital elevation models (DEM) and the average annual precipitation (AAP) databases in the study areas were established using ARC/INFO geographical information system (GIS) technology.
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