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All lentiviral shRNA studies were confirmed using at least two independent shRNA transductions and using at least one additional AKT1 and AKT2 shRNA sequence (Supplementary Figure 3).
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The specificity of the anti-IRS-1 and anti-erbB3 antibodies used in these studies was confirmed using rabbit immunoglobulin G antibodies.
The genome-wide representativeness of these 199 SNP markers for the whole genome in population genetic studies was confirmed using heterozygosity-heterozygosity correlation [ 48]: mean internal relatedness = 0.78, lower bound of 95% confidence intervals from 1000 bootstrap replicates (cil) = 0.444; mean homozygosity by loci = 0.86, cil = 0.596; standardised heterozygosity = 0.87, cil= 0.632.
In addition, the identities of all cell lines used in this study were confirmed using Identifiler® STR genotyping (Applied Biosystems).
The associations between gene profiles and survival detected in this study were confirmed using a three-fold approach.
In addition to literature review and LOOCV, the gene-survival associations detected in this study were confirmed using the information from the REMBRANDT database.
The quantitative analysis of the data obtained from the study was confirmed using Higuchi's kinetic model [24] to elucidate the mechanism of the drug release.
The identity of all cell lines used in this study was confirmed using Identifiler STR genotyping (Applied Biosystems, Foster, CA).
The heterogeneous particle size distribution observed in this study was confirmed using PDI analysis), which provides a measure of the broadness of the particle size distribution.
The stability of the [In]DTPA-MWNTs prior to and during the time course of the study was confirmed using TLC.
In further studies, these data were confirmed using freshly transduced primary BM cells to replicate the results obtained with HSC lines.
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