Synchronization efficiency in these studies was ascertained using flow cytometry and immunoblotting for cyclin A and PLK1.
The specificity of the antibody used in these studies was ascertained with Western blot and immunocytochemistry experiments performed in SW480 colon cancer cells transfected with TMIGD1 cDNA (Fig 7A and B).
The families used in this study were ascertained through the Kathleen Cuningham Foundation Consortium for Research into Familial Breast Cancer (kConFab).
Subjects in this study were ascertained as part of the Grady Trauma Project, which is an ongoing molecular genetic study with a primary focus on posttraumatic stress disorder.
Thus exposure data in this study were ascertained on all cases of cerebral palsy and a subset of all preterm infants enrolled in the longterm follow-up program.
The 107 additional CRA cases in our study were ascertained after recruitment to UKCAP was completed, and were therefore not considered for entry into the UKCAP study, thus, aspirin or NSAID use was not an exclusion criteria for these participants.
Dietary habits during the 6 months preceding enrolment in the study were ascertained through a validated food-frequency questionnaire covering frequency and quantity of consumption of about eighty food items and beverages [ 12].
Dietary habits during the 6 months preceding the woman's enrolment in the study were ascertained through a validated FFQ that covered the frequency of consumption and quantity of about eighty food items and beverages [ 26].
One of the challenges with such administrative database studies is ascertaining the reasons for colectomy.
The use of hair colorants in the twin study was ascertained via a questionnaire which first asked if subjects used hair colorants and then asked how long ago they had last used hair colorants (see SI Methods for further details).
