Parametric studies of substrate bias, deposition pressure, composition, and structure effects on the coatings' performance were conducted to determine the optimum deposition conditions.
In addition, high purity GroEL is essential for detailed studies of substrate-GroEL interactions as quantitative interpretation is impossible if the cavities are already occupied and blocked by other protein substrates present in the bacterial expression system.
Furthermore, structural and biochemical studies of substrate recognition by rNTrs will provide a rational foundation for therapeutic purposes.
Consequently experimental screening studies of substrate-supported nanoparticles of variable size are inevitable for finding the optimal size for particular tasks.
The actual RNase activity of SBL is essentially a pyrimidine base-specific RNase and detailed studies of substrate specificities have been performed [ 43].
For studies of substrate-induced conformational changes, cells or membranes were incubated with transport substrates (1 mM final concentration) at room temperature (25 °C for 10 min and 1 h respectively), followed by cross-linking and immunoblotting, as described above.
Intensive studies of substrate promiscuity in bacterial transporters and secretion systems will allow for the determination of a set of rules to which a transported compound needs to adhere.
To date, structural studies of substrate recognition within the BTB-Kelch family have been limited to KEAP1, including complexes with NRF2 [ 37, 38, 45], the degron motifs of prothymosin-α [ 46] and sequestosome-1 [ 47].
16 Mechanistic studies of substrate coupling between lipoxygenase (C-terminal) and catalase (N-terminal) have been inspired by the presence of this domain fusion in coral and other organisms.
These structures, together with spectroscopic studies of substrate binding to several OYEs, indicate that nitro-containing substrates can bind to OYEs in different binding modes, one of which leads to C=C double bond reduction and the other to nitro group reduction.
