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CPOH4 was cultivated at 20°C in preliminary studies (data not shown) revealed best growth at this temperature.
Our preliminary studies (data not shown) revealed that the pH optimum for the crude peroxidase is pH 6.0.
During pilot studies (data not shown), we observed that the best temperature to demonstrate a response was 43 °C.
Samples were assayed with iPLEX® Gold MassARRAY® 31-SNP and 27-SNP multiplex assays, developed in previous studies (data not shown).
Preliminary studies (data not shown) have demonstrated that no sedimentation of iodine contrast media occurred within 45 min following the preparation of a phantom containing contrast material.
No effect of tea was found in 6 out of 7 studies (data not shown).
From these studies (data not shown), a Km value of 65 nM was estimated.
This dose of eliglustat tartrate was selected based on earlier pilot tolerability and efficacy studies (data not shown).
The demographic characteristics of the participants in both the Sub-studies were similar to the main studies (data not shown).
Pharmacokinetic studies (data not shown) determined the half life of r84 in mice to be approximately five days.
It was found however, that no significant effects on cell viability take place at any of the concentrations utilized in our studies (data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com