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For the immunohistochemical studies, brain sections from infected and uninfected mice were deparaffinized and rehydrated using descending ethanol gradients before further processing.
For expression studies, brain sections were from male animals ranging in age from 1 day old to 6 years old (kindly provided in part by Dr. B. Czéh).
For immunological studies, brain sections were processed for dual immunofluorescence (IF) using humanized monoclonal antibody D13 for detection of PrPres and rabbit anti-ASMA.
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To accomplish this, we performed immunohistological studies in brain sections from C57BL/6 moribund and non-moribund mice.
This tolerance was functionally relevant, as mice examined after receiving HuD immunizations or at various times after HuD CD8+ T cell adoptive transfer (9 days, 18 days, one month) did not develop evidence of neurologic dysfunction or abnormalities evident by immunohistochemical studies of brain sections.
We performed immunohistochemical studies on brain sections from ADAD cases using either an anti-APP C-terminal or an anti-APP N-terminal antibody.
What possible useful information could be gained from studying these brain sections, any more than the brain of Lenin (for which the infant Soviet Union built an entire Brain Institute) or Einstein (whose pathologist sectioned his brain and packed it into mayonnaise jars for his various friends), or the many other great or notorious individuals that have been so treated in the past?
In our studies, GLP-1R-rich areas were observed in in vitro studies of rat brain sections (Fig. 7).
Results of CLSM studies on adult brain sections clearly showed LDH and MCT to colocalize with COX in mitochondria (Figs. 1 6).
Immunohistochemistry studies on human brain sections were carried out using paraffin-embedded formalin-fixed sections from four idiopathic PD cases (55).
(B ) Immunofluoresence studies of Atxn1 154Q/+ brain sections using anti-Ataxin-1 (green) and F11G3 (red) confirms the ATXN1 identity of the amyloid oligomers.
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