In such studies, the expression data exclusively determines which genes are used in the analysis.
For the profiling study, the expression data were normalized by global median normalization before further analysis.
For protein- or gene-expression studies, the expression data for a given condition or treatment would correspond to a single attribute measured across a number of biomolecules.
Although this gene was not included in this study, the expression data for this gene was found to be negatively correlated with the proliferation metagene and positively correlated with the metagene at 3p21 (data not shown).
Several factors affect the percentage of overlapping with the predicted enhancers in the second study, such as the number of orthologous genes available in our study, the expression data and prior biological knowledge used in the second study, the false positives in each study.
Similar to those studies, we analyse the expression data in conjunction with miRNA target prediction algorithms in order to decrease false positives, reduce the effects of experimental noise and ultimately to increase the likelihood of finding direct miRNA targets.
We studied the expression of SCN5A in BCa array data, and tested the effect of the VGSC-blocking anticonvulsant phenytoin (5,5-diphenylhydantoin) on Na+ current, migration, and invasion in BCa cells.
For each developmental stage and organ studied we provide the expression data obtained by RT-qPCR representing pri-miRNA transcript levels.
In this respect, the global approach in our study to assess the expression data in the manifold of metastatic tumors, all in the screening phase by microarrays, in the validation phase by RT-qPCR and response validation by target protein expressions and their miRNA dependent regulation, as mentioned above, proved to be advantageous.
To study OS and DFS, the expression data of TWIST were divided by tertiles.
While retrieval systems based on meta-data already exist, data-driven approaches that retrieve studies based on similarities in the expression data itself have a greater potential of uncovering novel biological insights.
